Journal: Methods (San Diego, Calif.)

Article Title: Determining the fidelity of tRNA aminoacylation via microarrays

doi: 10.1016/j.ymeth.2016.09.004

Figure Lengend Snippet: The station shown is underneath a small plexiglass hood to prevent dust accumulation and also to control humidity during the printing process. During array printing, the Floating Pin Replicator dips its pins into the 384-well printer plate containing the tRNA probes. The Glass Slide Indexing System then guides the pins of the Floating Pin Replicator to apply the probes to precise locations on the array. The Glass Slide Replicator Wash & Blot station is used to wash the pins of the Floating Pin Replicator when new section of the 384-well printer plate is being utilized in order to prevent probe cross-contamination. The Replicator Pin Drier accelerates the pin drying after they are washed.

Article Snippet: First, the Floating Pin Replicator (V&P Scientific, VP478A) has 24 individual pins that dip into a 384-well plate containing the tRNA probes and transfers a small volume of diluted oligonucleotide solution (~10 nl) to the glass array.

Techniques: Control